FIGURE

Fig. 3

ID

ZDB-FIG-070621-5

Publication

Cheng et al., 2006 - The homeobox gene irx1a is required for the propagation of the neurogenic waves in the zebrafish retina

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Fig. 3

Alterations of cell proliferation, cell death and lamination in irx1a morphant retina. (A–C) Embryos injected with control MO; (D–F) embryos injected with irx1a MO; (G–I) rescue embryos injected with irx1a MO and irx1a mRNA. (A,C,G) Acridine orange fluorescent staining (lateral view of 48 hpf embryos, anterior to the left) showed that irx1a morphant retina had ectopic cell death in compare to control or rescue embryos. t-Test analysis show that the number of cell death in morphant is significant higher (J, *P≤0.005) than control. (B,E,H) Anti-phosphohistone H3 immunostaining for mitotic cells (Coronal section of 48 hpf embryos, ventral to the bottom). The ratio of proliferating cells in irx1a morphant retina (E) was significant higher (K, t-test, *P≤0.005) than control retina (B) but the rescue embryos (H) was comparable to the control. In 96 hpf, the lamination of the retina in irx1a morphant embryos (F) was disrupted but rescue embryos (I) showed normal lamination as control embryos (C). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Open arrowheads indicate the position of the optic stalk.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-irx1a WT + MO3-irx1a
Knockdown Reagents:	MO2-irx1a MO3-irx1a
Observed In:	cell death cell population proliferation retina
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 123(3), Cheng, C.W., Yan, C.H., Hui, C.C., Strähle, U., and Cheng, S.H., The homeobox gene irx1a is required for the propagation of the neurogenic waves in the zebrafish retina, 252-263, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.