FIGURE

Fig. 2

ID

ZDB-FIG-070621-4

Publication

Cheng et al., 2006 - The homeobox gene irx1a is required for the propagation of the neurogenic waves in the zebrafish retina

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Fig. 2

Ocular defects in irx1a morphants. (A,E) Dorsal view of the head region. Embryos were raised in a bright environment until 96 hpf. irx1a morphants (E) showed darker pigmentation than control morphants (A). (B,F) Lamination defects of irx1a morphant retinas revealed by propidium iodide staining. In the control morphant (B), the retina is segregated into three distinct nuclear layers and two plexiform layers. Large retinal ganglion cells can be found in the GCL, which project axons to the optic stalk (white arrowheads). In contrast, the laminar structure was disrupted and cells were uniform in size in the irx1a morphant retinas (F). By 48 hpf, pax6 expression is restricted to the GCL and the proximal INL of the control retinas (C), whereas pax6 is expressed in the entire irx1a morphant retina (G). (D,H) Lateral view of 48 hpf eye, anterior to the right, rx1 is expressed in CMZ in control retina (D) but is expressed in the entire irx1a morphant retina (H). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Black arrowheads indicate the position of the optic stalk. Asterisks (*) indicate the position of the choroid fissure.

Expression Data

Genes:	pax6a rx1
Fish:	WT WT + MO2-irx1a WT + MO3-irx1a
Knockdown Reagents:	MO2-irx1a MO3-irx1a
Anatomical Terms:	ciliary marginal zone retina retinal ganglion cell layer retinal inner nuclear layer
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-irx1a WT + MO3-irx1a
Knockdown Reagents:	MO2-irx1a MO3-irx1a
Observed In:	eye melanocyte melanosome retina retina layer formation whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 123(3), Cheng, C.W., Yan, C.H., Hui, C.C., Strähle, U., and Cheng, S.H., The homeobox gene irx1a is required for the propagation of the neurogenic waves in the zebrafish retina, 252-263, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.