FIGURE

Fig. 6

ID

ZDB-FIG-070219-9

Publication

Ernest et al., 2007 - Localization of anosmin-1a and anosmin-1b in the inner ear and neuromasts of zebrafish

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Fig. 6

Expression of anosmin-1b in neuromast sensory hair cells. 120 hpf embryos were subjected to double immunofluorescence with anti-anosmin-1b polyclonal (green) and anti-acetylated tubulin monoclonal (blue) antibodies combined to TRITC-conjugated phalloidin staining of actin (red). (A–D) Confocal imaging of a lateral line neuromast, performed according to the apico-basal axis of hair cells, revealed anosmin-1b accumulation in the central most sensory hair cells. Projection of the confocal sections demonstrated staining in the hair bundle and, more weakly, at the cell membrane. (E–H) Projection of transverse confocal sections of a neuromast showing the apico-basal distribution of anosmin-1b within hair cells, with a dense staining observed at the top portion of the stereocilia and a weaker signal at the cell membrane. Data are maximum intensity projections of the acquired z-stacks. Scale bar: 5 μm (A–H).

Expression Data

Gene:	anos1b
Fish:	WT
Anatomical Term:	hair cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Gene expression patterns : GEP, 7(3), Ernest, S., Guadagnini, S., Prevost, M.C., and Soussi-Yanicostas, N., Localization of anosmin-1a and anosmin-1b in the inner ear and neuromasts of zebrafish, 274-281, Copyright (2007) with permission from Elsevier. Full text @ Gene Expr. Patterns