Fig. 5

Expression of anosmin-1a in sensory hair cells of the neuromast. (A) Confocal sections of the three terminal neuromasts showing anosmin-1a staining (green) in the hair bundle and, at a lower level, in the body of sensory hair cells. Kinocilia were labeled with an anti-acetylated tubulin antibody (blue). (B–D) Higher magnification of a single neuromast revealed a strong anosmin-1a accumulation in the stereocilia of hair cells and, to a lesser extent, in the cell body and in the kinocilia (lower half) which was co-labeled using an anti-acetylated tubulin antibody. Data represent the maximum intensity projection of the z-sections of the acquired z-stacks (A) or maximum intensity projections of the acquired z-stacks (B–D). Scale bar: 20 μm (A), 10 μm (B–D).