Fig. 6

Fig. 6 Expression of anosmin-1b in neuromast sensory hair cells. 120 hpf embryos were subjected to double immunofluorescence with anti-anosmin-1b polyclonal (green) and anti-acetylated tubulin monoclonal (blue) antibodies combined to TRITC-conjugated phalloidin staining of actin (red). (A–D) Confocal imaging of a lateral line neuromast, performed according to the apico-basal axis of hair cells, revealed anosmin-1b accumulation in the central most sensory hair cells. Projection of the confocal sections demonstrated staining in the hair bundle and, more weakly, at the cell membrane. (E–H) Projection of transverse confocal sections of a neuromast showing the apico-basal distribution of anosmin-1b within hair cells, with a dense staining observed at the top portion of the stereocilia and a weaker signal at the cell membrane. Data are maximum intensity projections of the acquired z-stacks. Scale bar: 5 μm (A–H).