FIGURE

Fig. 6

ID

ZDB-FIG-070118-9

Publication

Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish

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Fig. 6

Loss of Myod up-regulated Myf5 expression, and vice versa, in the cranial muscle that migrated from the anterior somites. The expression of myf5 was increased in sternohyoideus (sh) primordia, fin bud (fb), and posterior hypaxial muscle (phm) that migrated from the anterior somites, when embryos received myod-morpholino oligonucleotide (MO; vs. B). This consequence was consistent in trunk myogenesis. There was weak myf5 expression in the trunk of wild-type embryos at 48 hpf (C). However, myf5 appeared predominantly in the dorsal (ds) and ventral (vs) parts of somites in the myod morphants (E), indicating that myodknockdown up-regulated the expression of myf5 both in sh, which originated from trunk somite, and in trunk muscle. Similarly, the expression of myod was also enhanced in the trunk of myf5 morphants, compared to wild-type embryos (D vs. F). The myogenin expression was similar to that of myf5 in myod morphants (E, G) and to that of myod in myf5 morphants (F, H).

Expression Data

Genes:	myf5 myod1 myog
Fish:	WT WT + MO1-myf5 WT + MO1-myod1
Knockdown Reagents:	MO1-myf5 MO1-myod1
Anatomical Terms:	epaxial myotome region hyoid muscle hypaxial myotome region pectoral fin musculature somite
Stage:	Long-pec

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Acknowledgments

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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.