FIGURE

Fig. 8

ID

ZDB-FIG-061215-11

Publication

Shi et al., 2006 - Zebrafish foxe3: Roles in ocular lens morphogenesis through interaction with pitx3

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Cell proliferation in the foxe3 morphant lens. Frozen sections from control and foxe3 morpholino-injected embryos were immunolabeled for PCNA, a marker for proliferating cells. The control lens exhibits very few PCNA-positive cells at 5 and 7 dpf (panels A and C, respectively; arrows). Large numbers of anti-PCNA-labeled cells are identified in the foxe3 morphant lens at these ages (panels B and D, arrows). Small groups of PCNA-expressing cells are identified in the retinal circumferential marginal zones of both control and morphant eyes (panels A–D, arrowheads). A region of PCNA-labeled cells extends from the iris across the lens in the 7 dpf foxe3 morphant (panel D, double arrow). In addition, a region of cell proliferation in the brain is identified in the 5 dpf foxe3 morphant section (panel B, asterisk). Scale bars = 50 μm. Abbreviations: PL, photoreceptor layer; INL, inner nuclear layer and GCL, ganglion cell layer.

Expression Data

Gene:	pcna
Fish:	WT WT + MO1-foxe3
Knockdown Reagent:	MO1-foxe3
Anatomical Term:	lens
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-foxe3
Knockdown Reagent:	MO1-foxe3
Observed In:	epithelial cell lens epithelial cell proliferation lens epithelium
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 123(10), Shi, X., Luo, Y., Howley, S., Dzialo, A., Foley, S., Hyde, D.R., and Vihtelic, T.S., Zebrafish foxe3: Roles in ocular lens morphogenesis through interaction with pitx3, 761-782, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.