Fig. 5

 Anterior segment dysgenesis and reduced focal adhesions. Histology and marker comparisons of 10 dpf wild-type, lens-ablated and bal^a69 mutant larvae reveal lens-independent ocular phenotypes. (A) Iridocorneal angle shows normal periocular cell differentiation in wild-type (left) and lens-ablated (middle) larvae. Although pigment cell differentiation in bal^a69 mutants (right) occurs, many cells appear hyperplastic (arrows) and the collagen rich stromal layer (arrowheads) is disorganized. (B) Development of the ciliary canal (red arrows) is initiated normally in wild-type (left), lens-ablated (middle), and bal^a69 mutant (right) larvae. (C) Cornea differentiation in wild-type (WT), lens-ablated (LA), and two bal^a69 mutants (bal^a69). Analysis showed both focal (left mutant) and more severe (right mutant) corneal dysplasias. (D) Immunoreactivity for activated phospho[Y³⁹⁷] FAK in 54 hpf wild-type eyes. Inset d shows a higher magnification of the boxed differentiating lens epithelium and cornea. (E) Immunoreactivity for activated phospho[Y³⁹⁷] FAK in 54 hpf bal^a69 mutant eyes. Inset e shows a higher magnification of the boxed dysgenic lens epithelium and cornea. Note the robust activated FAK staining in the plexiform layers of both wild-type and mutant eyes, but the lack of intense and punctate staining the neural retina margins, lens cells, and corneal endothelium in the bal^a69 eyes. (F) Immunoreactivity for Paxillin in 54 hpf wild-type eyes. Inset f shows a higher magnification of the boxed differentiating lens epithelium and cornea. (G) Immunoreactivity for Paxillin in 54 hpf bal^a69 mutant eyes. Inset g shows a higher magnification of the boxed dysgenic lens epithelium and cornea. Paxillin immunoreactivity, although strong in the ciliary margin, is diffuse and reduced in the cornea.