FIGURE

Fig. 2

ID

ZDB-FIG-050601-3

Publication

Oates et al., 2005 - Cooperative function of deltaC and her7 in anterior segment formation

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Fig. 2

Effect of reduction of deltaC function on somitogenesis and segment polarity. Gene expression patterns in PSM and trunk somites of embryos at 14 hpf (10 somites) are shown in dorsal view after flat mounting with anterior upwards. In each pair of panels (A–D, F, H–J), an uninjected control is on the left and an embryo coinjected with 1 ng/nL dlcMO3 and 2 ng/nL dlcMO4 is on the right. In panels (E) and (G), the uninjected embryo is on the left (a) and embryos injected with dlcMOs (b), her7MOs (c; 2 ng/nL her7MO1 and 2 ng/nL her7MO2), or homozygous for the aei/dld mutation (d) are ordered to the right. Expression of myoD (A), fgf8 (B), fgfr4 (C), notch6 (D), notch5 (E), mespb (F), mespa (G), papc (H), ephA4 (I), and ephrinB2 (J) is shown. Brackets in panels (Ed) and (I) and arrow in panel (Ec) indicate affected regions, asterisks in panel (E) mark expression in overlying neural plate.

Expression Data

Genes:	efnb2a epha4a fgf8a fgfr4 mespaa mespba myod1 notch2 notch3 pcdh8
Fish:	WT dld^tr233/tr233 WT + MO1-her7 + MO2-her7 WT + MO3-dlc + MO4-dlc
Knockdown Reagents:	MO1-her7 MO2-her7 MO3-dlc MO4-dlc
Anatomical Terms:	neural keel segmental plate somite
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 280(1), Oates, A.C., Mueller, C., and Ho, R.K., Cooperative function of deltaC and her7 in anterior segment formation, 133-149, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.