Fig. 6

Analysis of the interaction between deltaC and her7. Myotome boundaries in the trunk are shown in 26 hpf embryos in lateral view with anterior to the left and dorsal upwards (A, B). (A) Wild type, uninjected control, (B) injected with 3 ng/nL her7MO1 and 3 ng/nL her7MO2. (C) Histogram comparing ALD in populations of embryos injected with increasing amounts of combined her7MOs, where the two numbers (e.g. 1 + 1) in inset legend are the concentration of her7MO1 and her7MO2 morpholino, respectively in ng/nL. Embryos injected with doses higher than 3 + 3 of combined morpholinos exhibited necrosis and non-specific defects. (D) Wild type embryo injected with 2 ng/nL her7MO1 and 2 ng/nL her7MO2, (E) embryo injected with 1 ng/nL dlcMO3 and 2 ng/nL dlcMO4, (F) embryo injected with her7- and dlcMOs combined. Arrows in panels (A), (B), and (D–F) indicate Anterior Limit of Defects (ALD) in these embryos. (G) Histogram comparing ALD in populations of embryos injected with the treatments in panels (D–F). (H–O) Expression of the her1 cyclic gene after injection of dlc- and her7MOs. Presomitic mesoderm of embryos at 80% epiboly (H, J) and bud (I, K) in whole mount. Comparison of coherent wavelike expression domains of her1 expression in wild type (H, I) and her7- and dlcMO-injected embryos (J, K). In each panel (H, I), two wild type embryos with representative phases of cyclic gene expression patterns are shown. In contrast, her7- and dlcMO-injected embryos are indistinguishable from each other (J, K). (L–O) Expression of the cyclic gene her1 in the PSM of embryos at 14 hpf (10 somites) in dorsal view after flat mounting with anterior up. (L) Two representative phases of cyclic her1 expression in wild type, control embryo; (M) comparison of levels and patterns of her1 expression after injection of dlcMOs (1 ng/nL dlcMO3 and 2 ng/nL dlcMO4), (N) dlc- and her7MOs at the concentrations mentioned, (O) her7MOs (2 ng/nL her7MO1 and 2 ng/nL her7MO2). For the treatments shown in panels (M–O), embryos in a batch were indistinguishable from each other.