CRISPR

CRISPR2-tfec

ID

ZDB-CRISPR-171108-1

Name

CRISPR2-tfec

Previous Names

None

Target

tfec (1)

Sequence

5' - GACGATCCTCAAGGCCTCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ba6	tfec
vc58	tfec
vc60	tfec
vc64	tfec

Expression

Gene expression in Wild Types + CRISPR2-tfec

No data available

Phenotype

Phenotype resulting from CRISPR2-tfec

No data available

Phenotype of all Fish created by or utilizing CRISPR2-tfec

Phenotype	Fish	Conditions	Figures
dorsal larval melanophore stripe iridophore tfec expression decreased amount, abnormal	tfec^ba6/ba6	standard conditions	Fig 5 from Petratou et al., 2021
trunk ventral region tfec expression decreased amount, abnormal	tfec^ba6/ba6	standard conditions	Fig. 3 from Petratou et al., 2018
eye iridophore absent, abnormal	tfec^ba6/ba6	standard conditions	Fig 3 from Petratou et al., 2021
iridoblast ltk expression absent, abnormal	tfec^ba6/ba6	standard conditions	Fig 5 from Petratou et al., 2021
eye tfec expression decreased amount, abnormal	tfec^ba6/ba6	standard conditions	Fig. 3 from Petratou et al., 2018
ventral larval melanophore stripe iridophore absent, abnormal	tfec^ba6/ba6	standard conditions	Fig 3 from Petratou et al., 2021
iridoblast ltk expression absent, abnormal	tfec^ba6/ba6	standard conditions	Fig. 3 from Petratou et al., 2018
iridophore pnp4a expression absent, abnormal	tfec^ba6/ba6	standard conditions	Fig. 4 from Petratou et al., 2018
iridophore pnp4a expression absent, abnormal	tfec^ba6/ba6	standard conditions	Fig 5 from Petratou et al., 2021
trunk dorsal region tfec expression decreased distribution, abnormal	tfec^ba6/ba6	standard conditions	Fig. 3 from Petratou et al., 2018
melanoblast mitfa expression absent, abnormal	tfec^ba6/ba6	standard conditions	Fig 5 from Petratou et al., 2021
iridoblast tfec expression decreased amount, abnormal	tfec^ba6/ba6	standard conditions	Fig 5 from Petratou et al., 2021
trunk medial region pnp4a expression decreased amount, abnormal	tfec^ba6/ba6	standard conditions	Fig. 4 from Petratou et al., 2018
iridoblast pnp4a expression decreased amount, abnormal	tfec^ba6/ba6	standard conditions	Fig 5 from Petratou et al., 2021
melanoblast mitfa expression decreased amount, abnormal	tfec^ba6/ba6	standard conditions	Fig 5 from Petratou et al., 2021
iridophore ltk expression absent, abnormal	tfec^ba6/ba6	standard conditions	Fig. 3 from Petratou et al., 2018
trunk dorsal region pnp4a expression decreased amount, abnormal	tfec^ba6/ba6	standard conditions	Fig. 4 from Petratou et al., 2018
dorsal larval melanophore stripe iridophore absent, abnormal	tfec^ba6/ba6	standard conditions	Fig 3 from Petratou et al., 2021
trunk dorsal region pnp4a expression decreased distribution, abnormal	tfec^ba6/ba6	standard conditions	Fig. 4 from Petratou et al., 2018
retinal pigmented epithelium color, abnormal	tfec^vc60/vc60	standard conditions	Fig. S1 from Sinagoga et al., 2020
eye decreased size, abnormal	tfec^vc60/vc60	standard conditions	Fig. S1 from Sinagoga et al., 2020
yolk larval melanophore stripe iridophore absent, abnormal	tfec^vc60/vc60	standard conditions	Fig 3 from Petratou et al., 2021
xanthoblast aox5 expression decreased distribution, abnormal	tfec^vc60/vc60	standard conditions	Fig 5 from Petratou et al., 2021
eye iridophore absent, abnormal	tfec^vc60/vc60	standard conditions	Fig 3 from Petratou et al., 2021
head iridophore absent, abnormal	tfec^vc60/vc60	standard conditions	Fig 3 from Petratou et al., 2021
dorsal larval melanophore stripe iridophore absent, abnormal	tfec^vc60/vc60	standard conditions	Fig 3 from Petratou et al., 2021
xanthoblast gch2 expression decreased distribution, abnormal	tfec^vc60/vc60	standard conditions	Fig 5 from Petratou et al., 2021
ventral larval melanophore stripe iridophore absent, abnormal	tfec^vc60/vc60	standard conditions	Fig 3 from Petratou et al., 2021
trunk dorsal region tfec expression absent, abnormal	foxd3^zdf10/zdf10; tfec^vc60/vc60	standard conditions	Fig 7 from Petratou et al., 2021
retinal pigmented epithelium eye pigmentation decreased magnitude, abnormal	mitfa^w2/w2; tfec^vc60/+	standard conditions	Fig. 2 from Sinagoga et al., 2020
retinal pigmented epithelium eye pigmentation decreased magnitude, exacerbated	mitfa^w2/w2; tfec^vc60/vc60	standard conditions	Fig. 2 from Sinagoga et al., 2020
optic fissure basement membrane increased amount, abnormal	mitfa^w2/w2; tfec^vc60/vc60	standard conditions	Fig. 1 from Sinagoga et al., 2020
optic fissure open, abnormal	mitfa^w2/w2; tfec^vc60/vc60	standard conditions	Fig. 1 from Sinagoga et al., 2020
retinal pigmented epithelium color, abnormal	mitfa^w2/w2; tfec^vc60/vc60	standard conditions	Fig. 2 from Sinagoga et al., 2020
periocular mesenchyme cranial neural crest cell decreased amount, abnormal	mitfa^w2/w2; tfec^vc60/vc60; w47Tg	standard conditions	Fig. 2 from Sinagoga et al., 2020
optic cup migratory neural crest cell decreased amount, abnormal	mitfa^w2/w2; tfec^vc60/vc60; w47Tg	standard conditions	Fig. 2 from Sinagoga et al., 2020
neural tube migratory neural crest cell mislocalised, abnormal	mitfa^w2/w2; tfec^vc60/vc60; w47Tg	standard conditions	Fig. 2 from Sinagoga et al., 2020

Citations