FIGURE

Fig. 2

ID

ZDB-FIG-200727-15

Publication

Sinagoga et al., 2020 - Mitf-family transcription factor function is required within cranial neural crest cells to promote choroid fissure closure

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Fig. 2

mitfa−/−;tfec−/− mutants display RPE and cNCC phenotypes. (A) At 48 hpf, mitf−/−;tfec+−/− and mitfa−/−;tfec−/− mutants display mild to severe hypopigmentation, respectively. The RPE eventually becomes normally pigmented by 4 dpf in both genotypes. (B) At 24 hpf, fewer cNCCs are present around the eye of mitfa−/−;tfec−/−;mitfa:GFP embryos than in the control (1) (***P=0.0007). However, mitfa−/−;tfec−/− mutants contain cNCCs posterior to the optic field (2). (C) Serial sections of mitfa−/−;tfec+/+;mitfa:GFP and mitfa−/−;tfec−/−;mitfa:GFP embryos at 48 hpf demonstrate that mitfa−/−;tfec−/− mutants possess fewer cNCCs in the POM surrounding the eyes. Dorsal is upwards in the images in B,C. Data are mean±s.e.m. Scale bars: 100 μm in A; 50 μm in B,C.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2 mitfa^w2/w2; tfec^vc60/+ mitfa^w2/w2; tfec^vc60/vc60 mitfa^w2/w2; tfec^vc60/vc60; w47Tg
Observed In:	neural tube migratory neural crest cell optic cup migratory neural crest cell periocular mesenchyme cranial neural crest cell retinal pigmented epithelium retinal pigmented epithelium eye pigmentation
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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