FIGURE SUMMARY
Title

Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish

Authors
Duan, M., Guo, X., Chen, X., Guo, M., Xu, H., Hao, L., Wang, C., Yang, Y.
Source
Full text @ Toxics
Fig. 1

Figure 1. Effects of CyB on the growth and development of adult zebrafish after 150 days. (A) K = weight (g)/length3(cm) × 100. (B) HSI = liver weight × 100/total weight. (C) BSI = brain weight × 100/total weight. weight. (D) GSI = gonad weight × 100/total weight. No significant differences were measured between control and exposure groups (p < 0.05).

Fig. 2

Figure 2. Effects of CyB on the fertility of zebrafish. (A) Cumulative average number of eggs per female. (B) Average number of spawning eggs per female and per week. (C) Rate of unfertilized eggs during the 150 d of exposure. The rate of unfertilized eggs was calculated as the cumulative number of unfertilized eggs × 100/total spawning eggs. Values are shown as the mean ± SD of three replicates per treatment (* p < 0.05; ** p < 0.01; *** p < 0.001).

Fig. 3

Figure 3. Histological observation of the gonads of zebrafish exposed to CyB for 150 d. Females: (A) control; (B) 0.1 µg/L; (C,C1) 1 µg/L; (D,D1) 10 µg/L. The oocytes in the ovaries included perinucleolar oocytes (PO), cortical alveolar oocytes (CO), early vitellogenic oocytes (EV) and late vitellogenic oocytes (LV). ((A–D) 200× magnification; (C1,D1) 400× magnification). Males (E) control; 0.1 µg/L; 1 µg/L; 10 µg/L. The spermatocytes included spermatogonia (Sg), spermatocytes (Sc), spermatids (St) and spermatozoa (Sz), Red arrows indicate a widened interstitial space (400× magnification). Percentage (%) of different stages of oocytes in females (F) and spermatogenic cells in males (G). The results are presented as the mean ± SD of three replicates (* p < 0.05; ** p < 0.01).

Fig. 4

Figure 4. Effects of CyB exposure for 150 d on sex steroid hormones and VTG in F0 zebrafish. (A) 17β-estradiol (E2), (B) testosterone (T), (C) 11-ketotestosterone (11-KT) and (D) vitellogenin. The results are presented as the mean ± SD of three replicates (* p < 0.05; ** p < 0.01).

Fig. 5

Figure 5. Transcription of HPGL axis-related genes in F0 zebrafish after 150 days of exposure to CyB. Females: (A) brain, (C) liver, (E) gonads; Males: (B) brain, (D) liver, (F) gonads. The results are presented as the mean ± SD of three replicates (* p < 0.05; ** p < 0.01).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Toxics
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.