Expression of the majority of the PcG genes that were tested is enriched in the adult germ line.

(A) Relative expression assessed by RT-qPCR for ezh2, eed, suz12a, rnf2, bmi1a, pcgf6, and rbbp4 and for the lower expressed genes ezh1, suz12b, pcgf5a, and pcgf5b in the adult ovary and testis and two somatic tissues: muscle and eye. Relative expression to the reference gene rsp18 is shown. Data based on three biological replicates and three technical replicates. Error bars indicate standard deviation. (B) Spatio-temporal expression of ezh2, ehz1, eed, suz12a, phc2a, rbbp4, rnf2, pcgf1, bmi1a, pcgf5a, pcgf5b, and pcgf6 in adult ovaries. Lower right panel shows a schematic representation of stage IB, II, and III of oogenesis. Stages of oogenesis are assessed according to Selman et al. [32]. Scale bar: 100 μm. (C) Expression of the PcG genes that were tested in different stages of oogenesis. Purple boxes indicate expression of the corresponding gene at that stage of oogenesis. Stages of oogenesis are assessed according to Selman et al. [32]. (D) Spatio-temporal expression of ezh2, eed, suz12a, phc2a, and bmi1a in adult testes. Lower right panel shows examples of the four stages of spermatogenesis. Scale bar: 100 μm. (E) Expression of the PcG genes that were tested in different stages of spermatogenesis. Purple boxes indicate positive expression of the corresponding gene at that stage of spermatogenesis. Stages of spermatogenesis are determined according to Leal et al. [33].

Expression of PRC2 members at early stages of embryonic development.

Spatio-temporal expression assessed by whole mount in situ hybridization of ezh2, ezh1, eed, suz12a, phc2a and rbbp4, at the 2-cell stage (0.75 hpf) and 50% epiboly (5.3 hpf). Scale bar: 200 μm.

Expression of PRC2 members at 1 dpf.

Lateral view of the spatio-temporal expression assessed by whole mount in situ hybridization of the PRC2 members: ezh2, ezh1, eed, suz12a, phc2a, and rbbp4, at 1 dpf zebrafish embryos.

Expression of PcG genes at 2 and 3 dpf.

(A) Spatio-temporal expression assessed by whole mount in situ hybridization of ezh2, ezh1, eed, suz12a, phc2a, rbbp4, and pcgf6 at 2 dpf. Lateral views are shown for all genes. Dorsal views of ezh2, ehz1, eed, phc2a, and rbbp4. Ventral view of pcgf6. (B) Spatio-temporal expression assessed by whole mount in situ hybridization of ezh2, ezh1, eed, phc2a, rbbp4, bmi1a, and pcgf6 at 3 dpf. Lateral views are shown for all genes. Dorsal views of ezh2, ezh1, eed, phc2a, and rbbp4. in: intestine, pf: pectoral fin (buds), HB: hindbrain, MHB: mid-hind brain boundary, pep: presumptive epiphysis, pa: pharyngeal arches 3–7, ot: optic tectum, re: retina, ob: olfactory bulb, n.d. = no data.

Expression of PcG genes in developing gonads.

(A) Spatio-temporal expression assessed by whole mount in situ hybridization of ezh2, eed, suz12a, phc2a, and bmi1a at 4 weeks post fertilization. (B) Expression patterns of ezh2, eed, suz12a, phc2a, and bmi1a, in gonads at 4 weeks post fertilization. Purple boxes indicate expression of the corresponding gene at that stage of oogenesis. (C) Spatio-temporal expression assessed by whole mount in situ hybridization at 5 weeks post fertilization of ezh2, eed, suz12a, phc2a, bmi1a, and the germ cell marker vasa. (D). Expression of ezh2, eed, suz12a, phc2a, and bmi1a, at 5 weeks post fertilization. Purple boxes indicate expression of the corresponding gene at that stage of oogenesis. Stages of oogenesis are assessed according to Selman et al. [32].