Fig. 1

Expression of the majority of the PcG genes that were tested is enriched in the adult germ line.

(A) Relative expression assessed by RT-qPCR for ezh2, eed, suz12a, rnf2, bmi1a, pcgf6, and rbbp4 and for the lower expressed genes ezh1, suz12b, pcgf5a, and pcgf5b in the adult ovary and testis and two somatic tissues: muscle and eye. Relative expression to the reference gene rsp18 is shown. Data based on three biological replicates and three technical replicates. Error bars indicate standard deviation. (B) Spatio-temporal expression of ezh2, ehz1, eed, suz12a, phc2a, rbbp4, rnf2, pcgf1, bmi1a, pcgf5a, pcgf5b, and pcgf6 in adult ovaries. Lower right panel shows a schematic representation of stage IB, II, and III of oogenesis. Stages of oogenesis are assessed according to Selman et al. [32]. Scale bar: 100 μm. (C) Expression of the PcG genes that were tested in different stages of oogenesis. Purple boxes indicate expression of the corresponding gene at that stage of oogenesis. Stages of oogenesis are assessed according to Selman et al. [32]. (D) Spatio-temporal expression of ezh2, eed, suz12a, phc2a, and bmi1a in adult testes. Lower right panel shows examples of the four stages of spermatogenesis. Scale bar: 100 μm. (E) Expression of the PcG genes that were tested in different stages of spermatogenesis. Purple boxes indicate positive expression of the corresponding gene at that stage of spermatogenesis. Stages of spermatogenesis are determined according to Leal et al. [33].