ZFIN Image: Xu et al., 2022, Figure 5

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Figure 5

Apoptosis and cell cycle arrest in zebrafish larvae caused by Cd²⁺ are mediated by Wnt signaling pathway. (A,B) Flow cytometry analysis and distribution of cell cycle phase of neurons and neuronal precursor cells. (C,D) Vertical view in the brain of AO-stained zebrafish larvae (6 dpf) and statistics of apoptotic cell numbers. (E) Relative expression analysis of genes related to cell apoptosis. In histograms, values are represented as mean ± SEM, while in boxplots, values are represented as median and mean, and whiskers signify 90% confidence interval. The results of nonparametric test and ANOVA, as well as multiple comparison test are listed in Table S2. “*” indicates significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001).

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Acknowledgments

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