ZFIN Image: Giusti et al., 2019, Fig. 2

Image

Figure Caption

Fig. 2 Overstimulation of the pituitary-thyroid axis in 120 hpf hypothyroid duox sa13017 mutant larvae (A) Schematic representation of the pituitary-thyroid axis with the thyroid hormones (TH)-mediated negative feedback loop inhibiting thyrotropin (TSH) secretion. (B) Whole-mount in situ hybridization (WISH) showed that larvae presenting a goitrous phenotype have increased tshb mRNA expression in the pituitary gland (arrow heads) compared to unaffected siblings. Images at the top show dorsal views of whole embryos (anterior to the left, scale bars: 100 μm). Images at the bottom show higher magnification views of the pituitary in coronal sections (anterior to the left, scale bars: 50 μm). (C) At the thyroid level (arrow heads), increased expression of TSH-dependent functional genes (tg, slc5a5/nis, tpo, duox and duoxa) was detected by WISH in larvae with a goitrous thyroid. Head region of representative larvae are shown in ventral views (anterior to the left, scale bars: 100 μm). (D) Fold change (mean ± SD, n = 3) of tg, slc5a5/nis, tpo and tshb mRNA expression in goiter affected animals compared to normal (WT or heterozygous mutants) specimens.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Molecular and Cellular Endocrinology, 500, Giusti, N., Gillotay, P., Trubiroha, A., Opitz, R., Dumont, J.E., Costagliola, S., De Deken, X., Inhibition of the thyroid hormonogenic H₂O₂ production by Duox/DuoxA in zebrafish reveals VAS2870 as a new goitrogenic compound, 110635, Copyright (2019) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.