ZFIN Image: Li et al., 2019, Fig. 4

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Fig. 4

NSCs in embryos injected with sox19b MO were prematurely differentiated. a Quantitative RT-PCR analysis for Ngn1, ascl1a, HuC, lslet1, and her3. Compared with the control group, the mRNA levels of Ngn1, ascl1a, HuC, and lslet1 in Sox19b MO-injection embryos increased significantly, but the mRNA level of her3 decreased significantly. b The expression levels of Ngn1, HuC, and lslet1 were detected by whole-mount in situ hybridization. Bar graphs show the percentage of normal embryos in each group. Data represent the mean of at least three independent experiments ± SD. *P < 0.05, **P < 0.01 versus control. c Quantitative RT-PCR analysis is shown for p21, p27, and p57. Knockdown of Sox19b caused a significant increase in the mRNA levels of cyclin-dependent kinase inhibitor. Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control. d Control and Sox19b MO-injected embryos were analysed by immunofluorescence staining of PHH3 (a marker of mitosis, red). Data represent the mean of at least three independent experiments ± SD. *P < 0.05 versus control

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Acknowledgments

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