FIGURE 4

FIGURE 4

Temporal configuration of mitochondrial biogenesis, fusion, fission, mitophagy and transport through embryogenesis. (A) Representative pictures illustrate the different stages of zebrafish development used for quantitative RT-PCR. (B) Gene expression of key mitochondrial actors in whole embryos or dissected somites. Values are estimated using the 2^–ΔΔCT method. Gene expression is normalized to 18S and compared to adult zebrafish muscle (bars are mean ± SEM). (C) Confocal imaging of mitochondrial network (Tomm20, T20; green) counterstained with Phalloidin (Ph; red) and Hoescht (H; blue) at 28 hpf in presence or absence of the fission inhibitor MDIVI-1 incubated since 24 hpf. (D) Confocal imaging of mitochondrial network (Tomm20, T20; green) counterstained with Phalloidin (Ph; red) and Hoescht (H; blue) at 28 hpf in embryos injected with mRNA encoding for the dominant negative DRP1 or with a mock at the one-cell stage zygote.