Fig. 4
Fig. 4
Vitellogenesis in esr1 deficient female zebrafish. (A) LV stage follicles were filled with vitellogenic granules in wild-type zebrafish. (B) Vitellogenic granules were found in the lateral part of LV stage follicles in esr1 mutants. (C) FG stage follicles were filled with big full vitellogenic granules in wild-types. (D) Only small vitellogenic granules were found in FG stage follicles of esr1 mutants. (E,F) Expression level of seven vtg genes at 90 dpf (E) and 180 dpf (F). The mRNA levels were normalized to ef1a. Average mRNA levels in ovaries of wild-types were defined as 1.0. (*P < 0.05; ***P < 0.001; Mean ± SEM).