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Fig. S2
npnt is expressed in sub-regions of arch epithelia, and fbn2b is expressed in arch mesenchyme. In situ hybridization of sections from the head of 60 hpf wild-type embryos. (A) fras1 is expressed in epithelia. (B) npnt is expressed in a subset of these epithelia, while (C) fbn2b is expressed in a complementary pattern in arch mesenchyme. (D) Illustration of section level. Ectoderm is shown in blue; cartilages are green; anterior endoderm is orange; posterior endoderm is red. Anterior-posterior level for 1, 2, 3, 4 and 5 are shown with lines in D. Scale bar is 50 µm, applicable to A-C.
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Reprinted from Developmental Biology, 416(1), Talbot, J.C., Nichols, J.T., Yan, Y.L., Leonard, I.F., BreMiller, R.A., Amacher, S.L., Postlethwait, J.H., Kimmel, C.B., Pharyngeal morphogenesis requires fras1-itga8- dependent epithelial-mesenchymal interaction, 136-48, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.