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Fig. 8 Midbrain and pretectal gene expression is altered in aus mutant embryos. Lateral (A,B,E-J) and dorsal (C,D) views of prim- 12 stage wild-type (A,C,E,G,I) and aus mutant (B,D,F,H,J) embryos. (A,B) noi (pax2.1). Expression is slightly expanded in the midbrain of the aus mutant embryo, particularly in ventral regions. (C,D) En. Expression appears expanded in the midbrain of the aus mutant embryo. (E,F) wnt1. Levels of transcripts are enhanced in the caudal diencephalon and mhb of the aus mutant embryo. Expression also appears to be increased in the dorsal hindbrain. (G,H) eph-A-rtk7. Pretectal expression (arrowhead) of eph-A-rtk7 is reduced in the aus mutant embryo. (I,J) zash-1b. Pretectal expression (arrowhead) of zash-1b is reduced in the aus mutant embryo. Abbreviations: mhb, midbrain/hindbrain boundary (*); pt, pretectum; wt, wild type. Scale bar: 50 μm (A-F); 60 μm (G-J).