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Fig. 6 Increased neuronal precursors in Her8a knockdown embryos.
Proneural genes were upregulated in the morpholino injected embryos and this effect was rescued by co-injection with her8a mRNA. Proneuronal markers neurogenin1 (A–C) and zash1 (D–F) were analyzed by in situ hybridization. Embryos were injected with control morpholino (A and D), 8 ng of MO2 (B and E), or co-injection of 8 ng of MO2 with 480 pg her8a mRNA (C and F). The most dramatic phenotypes are indicated by arrows. Stages of embryos are 6-somite stage (A-C) and 16-somite stage (D–F). (G) qPCR analysis showing the levels of neurogenin1 and zash1 are both significantly increased in embryos injected with her8a morpholino. * p<0.05.