Fig. 2

Fig. 2 Hindbrain development in wnt-deficient embryos. A-D: Expression of dlA at 30 hours postfertilization (hpf) in a Df^w5 mutant (A), a Df^w5 mutant injected with wnt3a-morpholino oligomers (MOs) (B), a wild-type embryo coinjected with wnt3a-MO and wnt8b-MO (C), and a Df^w5 mutant coinjected with wnt3a-MO and wnt8b-MO (D). E,F: Expression of erm at 30 hpf in a wild-type embryo (E) and a Df^w5 mutant coinjected with wnt3a-MO and wnt8b-MO (F). The otic vesicles (ov) and optic tectum (tec) are indicated to serve as landmarks. G,H: Expression of ephA4 at 24 hpf in a wild-type embryo (G) and a Df^w5 mutant coinjected with wnt3a-MO and wnt8b-MO (H). I,J: Expression of hoxb1a at 30 hpf in a wild-type embryo (I) and a Df^w5 mutant coinjected with wnt3a-MO and wnt8b-MO (J). K,L: Anti-acetylated tubulin staining of reticulospinal neurons (rsn) at 24 hpf in a wild-type embryo (K) and a Df^w5 mutant coinjected with wnt3a-MO and wnt8b-MO (L). M,N: Immunostaining with zn8 antibody at 30 hpf in a wild-type embryo (M) and a Df^w5 mutant coinjected with wnt3a-MO and wnt8b-MO (N). The zn8 antibody recognizes a cell adhesion molecule related to DM-Grasp (Fashena and Westerfield, [1999]) and normally labels commissural neurons (cn), some cranial ganglia, pharyngeal arches, floor plate, and heart (Trevarrow et al., [1990]). Commissural neurons are not produced in the wnt-deficient embryo. O,P: Immunostaining with a combination of four antibodies, zrf1-zrf4 (Trevarrow et al., [1990]), was used to label radial glia (rg) at 30 hpf in a wild-type (O) and a Df^w5 mutant coinjected with wnt3a-MO and wnt8b-MO (P). Images show dorsal views with anterior to the top (A-N) or lateral views with anterior to the left (O,P). Numbers mark locations of the corresponding rhombomeres. Scale bar in P = 60 μm in O,P, 70 μm in A-D,G-N, 85 μm in E,F.