Fig. 9

Fig. 9 The twd^rw685 mutation does not reduce the Notch activity. (A-C) Expression of the Notch target gene her4 was examined by RT-PCR using cDNAs synthesized from the total RNA of the whole body of wild-type and twd^rw685 embryos at 27 and 35 hpf (A), and by in situ hybridization of the wild-type (B) and twd^rw685 mutant (C) embryos at 24 hpf. Dorsal views, rostral towards the left. (D-G) Rohan-Beard sensory neurons (RB), caudal primary motoneurons (CaP), middle primary motoneurons (MiP) and rostral primary motoneurons (RoP) were visualized by in situ hybridization against isl1 (D,E) or isl2 (F,G) at 25 hpf in wild-type (D,F) and twd^rw685 mutant (E,G) embryos. Lateral views, rostral towards the left. (H-K) The rhombomere boundaries were examined by in situ hybridization against radical fringe (rfg; H,I; lateral views, rostral towards the left) or wnt1 (J,K; dorsal views, rostral towards the left) at 24 hpf in wild-type (H,J) and twd^rw685 mutant (I,K) embryos. Arrowheads indicate the rhombomere boundaries.