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Fig. 3 tp53 mutant embryos lack up-regulation of key downstream target genes after γ irradiation. Genes involved in the tp53 regulatory (tp53, mdm2), cell-cycle checkpoint (p21), and apoptotic (bax) pathways were analyzed in homozygous, heterozygous, and wild-type embryos with (+) or without (-) γ irradiation at 24 hpf (16 Gray). Gene expression was assayed at 30 hpf by single-embryo RT-PCR. Results are representative of at least five experiments. β actin was used to control gene expression.
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