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Fig. 1 Myelination and neuronal differentiation in the PLL. (A–F) show whole mount preparations of 2.5–5 dpf zebrafish. (A) Mbp expression begins around 2.5 dpf in myelinating glial cells (arrow) immediately posterior (arrowhead) to the posterior lateral line ganglion (pllg). (B) Expression is activated in progressively more posteriorly situated cells as time progresses. By 3 dpf mbp-expressing cells (arrow) extend 5–7 somites posterior to the pllg. (C) By 5 dpf mbp (arrow) is expressed all along the PLL. (D) Mbp expression in the anterior lateral line and the hindbrain at 5 dpf. c—commissure. ht—hindbrain tract. (E) Plp1b expression in the PLL (arrow) begins around 4 dpf. (F) PLL axons (arrow) at 5 dpf labeled with anti-acetylated tubulin. Higher power view of inset shows axons extending ventrally to innervate individual neuromeres (arrowheads). Panels G, H are electron micrographs of the PLL of a 5 dpf larva showing a transverse section through the PLL. (H) High power view of inset in panel G. Note loose wraps (arrowheads) around the large diameter axon. Scale bars in panels A, B and inset in panel F 5 μm; in panel C 20 μm; in panels D–F 10 μm; in panel G 1 μm; in panel H 200 nm.
Reprinted from Developmental Biology, 297(1), Kazakova, N., Li, H., Mora, A., Jessen, K.R., Mirsky, R., Richardson, W.D., and Smith, H.K., A screen for mutations in zebrafish that affect myelin gene expression in Schwann cells and oligodendrocytes, 1-13, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.