ZFIN Image: Lee et al., 2006, Fig. 7

Image

Figure Caption

Fig. 7 Wnt8b and Lef1 act through the canonical Wnt pathway in the posterior hypothalamus. White circles outline posterior hypothalamus in whole-mount views, and black ovals outline posterior hypothalamus in cross-sections. (A,B) At 30 hpf, wnt8b (blue) and lef1 (red) show overlapping and adjacent expression in the posterior hypothalamus. (C-F) In wnt8b morphants, sox3, zash1a, dlx2 and isl1 expression are specifically reduced in the posterior hypothalamus at 30 hpf. (G-I) A transgenic reporter for ß-catenin-mediated transcription (TOPdGFP) shows mRNA expression in the posterior hypothalamus (red) at 24 hpf. In the posterior hypothalamus, wnt8b expression (blue-H) partially overlaps with gfp, whereas lef1 (blue-I) almost completely overlaps with gfp. (J-O) In TOPdGFP embryos, GFP protein (brown) is expressed in the posterior hypothalamus. At 24 hpf (J-L) and 30 hpf (M-O), this expression is absent in wnt8b and lef1 morphants.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development