Fig. 6

Increased social cohesion and anxiety-like behavior in Het adult rfc2 KO zebrafish. A: Representative image of the shoaling bowl assay. Each arrow indicates a single zebrafish. B: Quantification of DM of zebrafish during shoaling bowl assay. Compared to WT siblings, rfc2 KO Het zebrafish showed a significant reduction in their movement during the test. n = 5 for WT and n = 9 for Het fish in 15 times of tests. C: Quantification of MID between three subjects. Compared to WT siblings, rfc2 KO Het tend to stay closer each other. D: Heatmap of scototaxis test. The red color indicates increased dwell time and blue indicates decreased. E: Quantification of duration time in the light zone during scototaxis test. Compared to WT siblings, rfc2 KO Het zebrafish stayed more time in the dark zone than the light zone. n = 5 for WT and n = 9 for Het fish in total 13 times of tests. Data are presented as mean ± SEM. Statistical significance was determined by the Mann Whitney U test. ns, not significant, P > 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. MID, mean individual distance; Het, heterozygous; DM, distance moved; LZD, light zone duration; WT, wild-type; KO, knockout; Het, heterozygous; SEM, standard error of the mean.