Fig. 3

Reduced head and brain size in rfc2 KO and rfc5 KO zebrafish. A: Reduced head size in KO zebrafish is prominent at 5 dpf, whereas relatively comparable at 2 dpf. Eye size is also reduced in both KO zebrafish, compared to WT zebrafish at 5 dpf. n = 5 for rfc2 KO and n = 4 for rfc5 KO at 2 dpf. n = 13 for rfc2 KO and n = 25 for rfc5 KO at 5 dpf. Lateral view. B: Quantification of eye size in rfc2 KO and rfc5 KO zebrafish. The planar area of eye was measured by Image J software at 3 dpf and 5 dpf, respectively. C: Reduced brain size in rfc2 KO zebrafish at 5 dpf. Dorsal view. D: Quantification of reduced brain size (bordered area in C) in rfc2 KO zebrafish at 5 dpf. n = 18 for WT and n = 31 for KO. E: Representative fluorescent image of rfc5 KO zebrafish crossed with neuron-specific GFP transgenic line Tg(huc:egfp) at 5 dpf. Dorsal view, anterior is to the left. F: Quantification of brain size in rfc5 KO at 5 dpf. Relative brain size was obtained by multiplying the length of (a) and (b) marked in the brain image of (E). n = 7 for WT and n = 34 for KO. Data are presented as mean ± SEM. Statistical significance was determined by the Kruskal–Wallis tests with post-hoc Dunn's multiple comparisons tests. ns, not significant, P > 0.05; ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. Scale bar, 100 μm (A, C, E). dpf, days post fertilization; WT, wild-type; KO, knockout; SEM, standard error of the mean.