Acute expression of ap2s1 is sufficient for acoustic habituation (A) Timeline for acute induction of ap2s1-gfp expression in larvae. (B and C) 6 dpf larva carrying Tg(hsp70:ap2s1-gfp) without induction (B) and 5 h after a single 15-min 38°C heat shock treatment (C). The transgene is marked with an independent GFP marker in the heart (asterisk). Scale bar is 200μm. (D) Acoustic habituation percentage for sibling (blue) and ap2s1p172 (red) larvae in the final 10 of 40 intense (23.8 dB) stimuli at 1s ISI (stimuli 31–40), with or without Tg(hsp70:ap2s1-gfp) as indicated. All larvae were treated identically with a heat shock. ∗∗∗∗p < 0.0001, ∗∗p < 0.01, Bonferroni corrected one-tailed Mann-Whitney test. (E) Behavior selection bias of sibling (blue) and ap2s1p172 (red) larvae in response to weak stimuli (−8.2 dB). ∗∗p < 0.01, ns = not significant, Bonferroni corrected one-tailed Mann-Whitney test. (F‒H) Average kinematic parameters of SLC startle responses for sibling (blue) and ap2s1p172 (red) larvae: latency (F), maximal turning angle (G), and maximal angular velocity (H). ∗∗∗∗p < 0.0001, ns = not significant, Bonferroni-corrected one-tailed t-test with Welch’s correction. Data are represented as mean ± SEM in all panels.
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