Neuronal expression of ap2s1 is sufficient for acoustic habituation and response kinematics (A and B) 3 dpf non-transgenic larva (A) and larva carrying Tg(NBT:ap2s1-gfp) (B) expressing green fluorescence in the brain and spinal cord. Scale bar is 200μm. (C) Acoustic habituation percentage for sibling (blue) and ap2s1p172 (red) larvae in the final 10 of 40 intense (23.8 dB) stimuli at 1s ISI (stimuli 31–40), with or without Tg(NBT:ap2s1-gfp) as indicated. ∗∗p < 0.01, ∗∗∗∗p < 0.0001, Bonferroni corrected one-tailed Mann-Whitney test. (D) Behavior selection bias of sibling (blue) and ap2s1p172 (red) larvae in response to weak stimuli (−8.2 dB). ∗p < 0.05, ns = not significant, Bonferroni corrected one-tailed Mann-Whitney test. (E‒G) Average kinematic parameters of SLC startle responses for sibling (blue) and ap2s1p172 (red) larvae: latency (E), maximal turning angle (F), maximal angular velocity (G). ∗∗∗∗p < 0.0001, ns = not significant, one-tailed t-test with Bonferroni correction. Data are represented as mean ± SEM in all panels.
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