FIGURE

Fig. 8

ID

ZDB-FIG-240509-41

Publication

Tucker et al., 2024 - Zebrafish raptor mutation inhibits the activity of mTORC1, inducing craniofacial defects due to autophagy-induced neural crest cell death

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Loss of raptor increases autophagy. (A-C″) Confocal images of double-labeling experiment using 36 hpf transgenic CMV:EGFP-lc3 fish stained with lysotracker red. Single z-sections are shown, anterior is towards the left. White arrowheads indicate dual-positive puncta. Representative images of (A-A″) wild-type, (B-B″) heterozygous and (C-C″) raptor mutant fish. (D) Quantification of dual-positive puncta in fish exposed to DMSO (diamonds) or rapamycin (circles) from 6 to 36 hpf. Statistical comparisons were performed using two-sample t-tests with unequal variances assuming a significance level of α=0.05. (E) Quantification of dual-positive puncta in wild-type (circles), heterozygous (triangles) and raptor mutant (squares) fish. The statistical analyses were conducted using one-way ANOVA followed by Tukey's honest significant difference (HSD) post-hoc test for multiple comparisons, *P<0.05, **P<0.01, ***P<0.001. Data are mean±s.e.m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rptor^au93/au93; zf155Tg (AB)
Observed In:	pharyngeal arch autophagy
Stage:	Prim-25

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development