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Fig. 8 Loss of raptor increases autophagy. (A-C″) Confocal images of double-labeling experiment using 36 hpf transgenic CMV:EGFP-lc3 fish stained with lysotracker red. Single z-sections are shown, anterior is towards the left. White arrowheads indicate dual-positive puncta. Representative images of (A-A″) wild-type, (B-B″) heterozygous and (C-C″) raptor mutant fish. (D) Quantification of dual-positive puncta in fish exposed to DMSO (diamonds) or rapamycin (circles) from 6 to 36 hpf. Statistical comparisons were performed using two-sample t-tests with unequal variances assuming a significance level of α=0.05. (E) Quantification of dual-positive puncta in wild-type (circles), heterozygous (triangles) and raptor mutant (squares) fish. The statistical analyses were conducted using one-way ANOVA followed by Tukey's honest significant difference (HSD) post-hoc test for multiple comparisons, *P<0.05, **P<0.01, ***P<0.001. Data are mean±s.e.m.