FIGURE

Fig. 1

ID

ZDB-FIG-240509-34

Publication

Tucker et al., 2024 - Zebrafish raptor mutation inhibits the activity of mTORC1, inducing craniofacial defects due to autophagy-induced neural crest cell death

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Fig. 1

A mutant allele of regulatory-associated protein of mTOR (raptor) generated using CRISPR-Cas9. (A) The raptorau93 mutation is located in exon 7 (black arrowhead), upstream of all 12 known Raptor phosphorylation sites (red dots). (B) Sanger sequencing results for wild-type and raptorau93 alleles. The CRISPR-Cas9 gRNA target is indicated by a bracket. The 4 bp deletion is indicated by the black rectangle. (C) The raptor au93 allele is predicted to have altered amino acid sequence beginning at residue 231, with a predicted premature termination codon at residue 238 (red, asterisk). The CRISPR-Cas9 gRNA target is indicated by a bracket and the four deleted nucleotides are in bold and red.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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