|
Fig. 1 A mutant allele of regulatory-associated protein of mTOR (raptor) generated using CRISPR-Cas9. (A) The raptorau93 mutation is located in exon 7 (black arrowhead), upstream of all 12 known Raptor phosphorylation sites (red dots). (B) Sanger sequencing results for wild-type and raptorau93 alleles. The CRISPR-Cas9 gRNA target is indicated by a bracket. The 4 bp deletion is indicated by the black rectangle. (C) The raptor au93 allele is predicted to have altered amino acid sequence beginning at residue 231, with a predicted premature termination codon at residue 238 (red, asterisk). The CRISPR-Cas9 gRNA target is indicated by a bracket and the four deleted nucleotides are in bold and red.