Fig 3

syt7a mutant larvae do not exhibit altered behavioral phenotype at 6 days post fertilization.

(A) Acoustic startle short term habituation assay. i) Percent startle of both siblings (n = 20 larvae, black) and p432 homozygous mutant larvae (n = 9 larvae, red) at baseline (10 acoustic stimuli with 40 sec interstimulus interval), as well as during stimuli that induce habituation (acoustic stimulus presented with 1sec interstimulus interval). Average percent startle is reported for stimulus number 1–10, 11–20, 21–30. Any larvae that had an average probability of startle of less than 60% during baseline stimuli were omitted from habituation analysis. ii) Habituation index calculated for both siblings and p432 homozygous mutant larvae. Habituation index is calculated using the following formula: % Habituation = [1- ((%Startle Stimulus 21–30)/ (%Startle Baseline))] *100. Mann-Whitney test was used to compare siblings to p432 mutants (p = 0.7871). (B) Analysis of dark flash response for sibling larvae (n = 74 larvae) and p432 homozygous mutant larvae (n = 20 larvae). i) O-bend probability, ii) average O-bend latency and iii) average distance traveled during O-bend are shown. For statistical purposes, values were normalized to sibling responses, and student’s t-test was performed to compare mutants and sibling groups with Bonferroni for multiple comparisons of 86 parameters analyzed during behavioral testing (See S2 Fig for all parameters tested).