Figure 3

appb^−/− mutants have altered waking activity and sleep across the day-night cycle

Exemplar 48 h traces of average waking activity taken from a single experiment of appa^Δ5 mutants, heterozygous, and wild-type siblings (5-7dpf) on a 14h:10h light:dark cycle. Each line and shaded ribbon show the mean ± SEM.

(B) Day waking activity and (C) night waking activity for appb^−/− mutants and siblings from appb^+/− incrosses, combined across N = 5 experiments.

(D) Exemplar 48 h traces of average sleep for the same experiment shown in (A).

(E) Day sleep, (F) night sleep, (G) day sleep length, (H) night sleep length, (I) day sleep bout number, and (J) night sleep bout number of WT, heterozygous, and appb^−/− mutants normalized to WT siblings as in (B) and (C). At top, each dot is an animal normalized to the mean of their experimentally matched WT. At bottom, shown are the effect size ±95%CI relative to WT. ^nsp > 0.05, ∗p ≤ 0.05, ∗∗p ≤ 0.01, Kruskal-Wallis, Tukey’s post hoc test. n = number of larvae.