Figure 2

appa^Δ5/Δ5 mutants have reduced day waking activity but no sleep phenotype

Exemplar 48 h traces of average waking activity taken from a single experiment of appa^Δ5/Δ5 mutants, heterozygous, and wild-type siblings (5–7dpf) on a 14 h:10 h light:dark cycle. Each line and shaded ribbon show the mean ± SEM.

(B) Day waking activity and C) night waking activity for appa^Δ5/Δ5 mutants and siblings from appa^+/Δ5 incrosses, combined across N = 5 experiments. Each dot is a single larva, normalized to the mean of their experimentally matched WTs. At the bottom are plotted the effect sizes (±95% confidence interval [CI]) relative to WT.

(D) Exemplar 48 h traces of average sleep for the same experiment shown in (A).

(E) Day sleep and (F) night sleep of WT, heterozygous, and appa^Δ5/Δ5 mutants normalized to their WT siblings, as in (B) and (C). ^nsp > 0.05, ∗p ≤ 0.05, Kruskal-Wallis, Tukey’s post hoc test. n = number of larvae.