FIGURE

Fig. 4

ID

ZDB-FIG-231215-12

Publication

Zhang et al., 2022 - Blf and drl cluster synergistically regulate cell fate commitment during zebrafish primitive hematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

blf and the drl cluster regulate macrophage versus neutrophil fate choice during primitive myelopoiesis. (A) Left: RNA in situ hybridization of mpx, lyz, mpeg1, mfap4.1 and coro1a expression at 23 hpf. Right: The number of myeloid cells in different genotypes for each marker. Scale bars: 100 μm. (B) Left: Merged confocal images of the uncaged fluorescein and FISH signals in the trunk at 72 hpf. Scale bars: 50 μm. White arrowheads indicate the overlapping of fluorescein and FISH staining. Right: Quantification of fluorescein-labeled myeloid cells adopting the neutrophil or macrophage fate in control and blf−/−; drl 4KO embryos. Nmpx+, number of mpx-expressing cells; Nmpeg1+, number of mpeg1-expressing cells; NFlu+, number of fluorescein-labeled cells. Quantification was carried out on a 150 μm×275 μm field. Data were collected for 12-16 embryos per experiment group. n.s, not significant, *P<0.05, **P<0.01, ***P<0.001 (t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development