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NF-κB inhibition affect thyroid follicle formation in mice. a The changes of body weight in mice treated by IKK2 inhibitor TPCA1. Yellow inverted triangles in X axis indicated the administration time of TPCA1, and the body weight of mice were measured every three days. Repeated measures ANOVA used for significance testing. b Representative image show the body size of mice treated by TPCA1 under stereoscope. The serum levels of T3 (c), T4 (d) and TSH (e) in the mice after treated with TPCA1 for one month. f Representative image of histology analysis of thyroid tissues at postnatal day 30 mice after treated with TPCA1. g Statistical assessment of follicle numbers formed at the maximum sections of thyroid tissues in mice treated with TPCA1. h Representative images showing TPO and Thyroxine levels in thyroid tissues of the postnatal day 30 mice treated with or without TPCA1. i Statistical measurement of TPO and thyroxine positive lumens (indicated of functional maturation) in thyroid tissues of the postnatal day 30 mice treated with TPCA1. j The expression of NKX2-1 and E-cadherin in thyroid tissues of postnatal day 30 mice treated with TPCA1 were detected by IF. k Statistical assessment of the percentage of thyroid epithelial cells clustering into solid mass (with no lumen formed and no polarized E-cadherin expression) in thyroid tissues of postnatal day 30 mice treated with TPCA1. Scale bar, 50 μm. n = 8 biologically independent samples in (a, c, d, e, g, i, k). Two-sided Student’s t test used. Data are shown as mean ± SD. Source data are provided as a Source data file.
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