Fig. 6
- ID
- ZDB-FIG-231121-65
- Publication
- Zhang et al., 2023 - Novel biallelic variants in the PLEC gene are associated with severe hearing loss
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Analysis of plectin distribution in zebrafish. A-J’: Representative images of at least three fish at each time point. A-B: Otic vesicle from 1dpf wild type zebrafish embryo immunostained for plectin (red) and counterstained with phalloidin (green). Asterisks in B denote the hair cell precursors. A’-B’: High magnification of the framed area in A. Plectin localizes at the base of the hair cell bundle (A’). C-F: Apical (C-D) and basal (E-F) views of the medial crista from a 5dpf Tg(NeuroD-GFP). C-D: Plectin localizes at the base of the hair cell bundle (asterisk in D). E-F: The afferent fibers (GFP positive, AF) and the hair cell-neuron interface (asterisks) are also positive for plectin (red). E’-F”: High magnification Z-view images from E and F, showing the contacts between the neuronal fibers and the base of the hair cells (asterisks). G-J: Neuromast from a 5dpf Tg(NeuroD-GFP), showing apical localization of plectin (asterisks). G’-J”: High magnification images from frame in J showing expression of plectin by the neuronal fibers (asterisks) and at the interface between the hair cells and the neurons (arrowheads). K-L: Cartoons depicting a 5dpf neuromast (K, top view) and a crista (L, lateral view). Plectin (red). Phalloidin (green). GFP (green). Otoferlin (cyan). A: anterior, D: dorsal, st: stereocilia. Scale bars: A-D, A’-B’, E’-F’, E”-F”, G-J, G’-J’: 5 μm, E-F: 10 μm. |
Reprinted from Hearing Research, 436, Zhang, T., Xu, Z., Zheng, D., Wang, X., He, J., Zhang, L., Zallocchi, M., Novel biallelic variants in the PLEC gene are associated with severe hearing loss, 108831108831, Copyright (2023) with permission from Elsevier. Full text @ Hear. Res.