Fig. 6

Fig. 6 Analysis of plectin distribution in zebrafish. A-J’: Representative images of at least three fish at each time point. A-B: Otic vesicle from 1dpf wild type zebrafish embryo immunostained for plectin (red) and counterstained with phalloidin (green). Asterisks in B denote the hair cell precursors. A’-B’: High magnification of the framed area in A. Plectin localizes at the base of the hair cell bundle (A’). C-F: Apical (C-D) and basal (E-F) views of the medial crista from a 5dpf Tg(NeuroD-GFP). C-D: Plectin localizes at the base of the hair cell bundle (asterisk in D). E-F: The afferent fibers (GFP positive, AF) and the hair cell-neuron interface (asterisks) are also positive for plectin (red). E’-F”: High magnification Z-view images from E and F, showing the contacts between the neuronal fibers and the base of the hair cells (asterisks). G-J: Neuromast from a 5dpf Tg(NeuroD-GFP), showing apical localization of plectin (asterisks). G’-J”: High magnification images from frame in J showing expression of plectin by the neuronal fibers (asterisks) and at the interface between the hair cells and the neurons (arrowheads). K-L: Cartoons depicting a 5dpf neuromast (K, top view) and a crista (L, lateral view). Plectin (red). Phalloidin (green). GFP (green). Otoferlin (cyan). A: anterior, D: dorsal, st: stereocilia. Scale bars: A-D, A’-B’, E’-F’, E”-F”, G-J, G’-J’: 5 μm, E-F: 10 μm.