FIGURE

Figure 4

ID

ZDB-FIG-231113-25

Publication

Belcher et al., 2023 - The zebrafish paralog six2b is required for early proximal pronephros morphogenesis

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Figure 4

Pronephric marker expression in F₀ embryos following six2b CRISPR/Cas9 reaction mix injection. (a) Pronephric marker expression at 24 hpf. No significant changes in gene expression or tissue morphology were observed. (b) Expression of pronephric markers at 48 hpf. Morphological defects in the glomerulus and proximal tubule are observed following genetic disruption of the six2b locus. The proximal straight tubule marked by trpm7 appeared unaffected. Arrowhead for pax2a denotes the neck region. (c) PCR detection of genomic DNA lesions in the six2b gene from two independently injected embryos displaying defective wt1a expression domain morphology. Bottom arrow points to wild-type allele amplicon. (d) Representative TIDE analysis of F₀ CRISPR/Cas9 injected embryo following in situ hybridization for wt1a.

Expression Data

Genes:	cdh17 nphs1 nphs2 pax2a slc20a1a trpm7 wt1a
Fish:	AB AB + CRISPR1-six2b
Knockdown Reagent:	CRISPR1-six2b
Anatomical Terms:	pronephric glomerulus pronephric proximal convoluted tubule pronephric proximal straight tubule pronephric tubule pronephric tubule epithelium
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR1-six2b
Knockdown Reagent:	CRISPR1-six2b
Observed In:	pronephric glomerulus pronephric proximal convoluted tubule pronephric tubule
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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