FIGURE

Figure 2

ID

ZDB-FIG-231113-23

Publication

Belcher et al., 2023 - The zebrafish paralog six2b is required for early proximal pronephros morphogenesis

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Pronephric marker expression following injection of six2b MO1. (a) Expression of pronephric markers at 24 hpf following MO1 injection. No significant changes in gene expression or tissue morphology were observed. (b) Expression of pronephric markers at 48 hpf following MO1 injection. Knock-down of six2b resulted in glomerular defects as seen with wt1a, nphs1, and nphs2. Proximal tubule morphogenesis was defective using cdh17 and slc20a1a probes. The proximal straight tubule appeared unaffected using trpm7. (c) Compiled data of wt1a morphological defects following mouse Six2 mRNA injection alone or combined with MO1. Six2 mRNA co-injection partially rescued the MO1 induced wt1a phenotype. Arrowheads for 48 hpf pax2a expression denote neck region.

Expression Data

Genes:	cdh17 nphs1 nphs2 pax2a slc20a1a trpm7 wt1a
Fish:	AB AB + MO1-six2b
Knockdown Reagent:	MO1-six2b
Anatomical Terms:	pronephric glomerulus pronephric proximal convoluted tubule pronephric proximal straight tubule pronephric tubule pronephric tubule epithelium
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-six2b
Knockdown Reagent:	MO1-six2b
Observed In:	pronephric glomerulus pronephric proximal convoluted tubule pronephric tubule
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.