Fig. 2

Analysis of morphological parameters in wild-type and GLA-mutant mitochondria in cells from proximal and distal renal tubules. A Electron microscopy showing representative micrographs of mitochondria (red arrows) in cells from wild-type and mutant proximal and distal tubules; B Mitochondrial form factor and aspect ratio plotted against each other in proximal tubule; C Statistical comparison using mean with 95% confidence intervals (CI) for form factor and aspect ratios in mitochondria from proximal tubule cells; D Mitochondrial form factor and aspect ratio plotted against each other in distal tubule; E Statistical comparison using mean and 95% confidence intervals (CI) for form factor and aspect ratios in mitochondria from distal tubule cells; F, G Mitochondrial morphological parameters comparison in proximal tubules and distal tubules; H, I Mitochondrial volume and density determination in proximal and distal tubules. Values are represented as mean with upper 95% CI. Data were analyzed using Mann–Whitney test. *p-value ≤ 0.05; ****p-value ≤ 0.00001; ns = non-significant