FIGURE 3

Regulation of downstream hematopoietic lineages by hydroxysafflor yellow A (HSYA) in N,N,N′,N′‐tetrakis(2‐pyridylmethyl)ethylenediamine (TPEN)‐treated zebrafish: (A and B) representative images showing neutrophils in Tg(lyz:eGFP) embryos (A) and macrophages in Tg(mpeg:eGFP) embryos (B) in control and TPEN (150 μM)‐treated embryos with or without incubation of ZnSO₄ (100 μM) or HSYA (100 μg/ml); (C) quantitative result of neutrophils and macrophages; (D) whole in situ hybridization showing the transcriptional expression of cmyb and lyz in zebrafish embryos; (E) representative fluorescence images and quantitative results of red blood cells in Tg(LCR:eGFP) embryos on 4.5 dpf; (F) whole in situ hybridization showing the transcriptional expression of hbae3 in zebrafish embryos. The numbers at the top‐right corner of (D–F) show the number of embryos with representative phenotypes/the total number of embryos in the group. Positive stained cells are pointed by a black arrowhead in the zoomed insets of (D) and (F). At least 7 embryos were included in each group; Scale bar (in A, B, and E), 200 μm; scale bar (in D and F), 500 μm. hpf, hours post fertilization. *p < 0.05; **p < 0.01.