Figure 3

Reduced expression of usherin and Whrnb at the photoreceptor periciliary region of adgrv1^rmc22 zebrafish larvae. Retinal cryosections of wild-type and adgrv1^rmc22 zebrafish larvae (5 dpf) stained with antibodies directed against usherin (red) (A) or Whrnb (red) (B) and centrin (green). Nuclei are counterstained with DAPI (blue). (A): In wild-type larvae, usherin was present at the photoreceptor periciliary region in close proximity to the connecting cilium marker centrin. The intensity of the usherin signal in adgrv1^rmc22 retinal sections was significantly reduced when compared to wild-types (n = 7 adgrv1^rmc22 mutant larvae and n = 4 wild-type larvae). (B): In wild-type larvae, Whrnb was present at the photoreceptor periciliary region in close proximity to the connecting cilium marker centrin. The intensity of the Whrnb signal in adgrv1^rmc22 retinal sections was significantly reduced when compared to wild-types (n = 7 adgrv1^rmc22 mutant larvae and n = 6 wild-type larvae). Intensities of fluorescence signals were quantified (mean ± SD) and plotted in a scatter plot next to the corresponding pictures. **** indicates p < 0.0001 (two-tailed unpaired Student’s t-test). Scale bar: 10 μm.