Conditional overexpression of cmyc in ∆113p53+ cells promotes heart regeneration dependent on Glut1. (A) Immunostaining of MF20 (in red) and EDU incorporation assay (in green) of injury hearts at 14 dpa in WT and Tg(∆113p53:cmyc) zebrafish intraperitoneally injected with DMSO (the injection control) or WZB117. The nuclei were stained with DAPI (in blue). Framed areas were magnified in right panels. Scale bar: 50 μm or 20 μm as indicated. (B) Immunostaining of MF20 (in red) of injury hearts at 14 dpa in WT and Tg(∆113p53:cmyc) zebrafish intraperitoneally injected with DMSO (the injection control) or WZB117. The nuclei were stained with DAPI (in blue). Scale bar: 200 μm. (C) Statistical analyses of EDU+ CMs in (A). The EDU+ CMs were presented as the percentage of the total MF20+ cells at the injury sites. Each dot represents an individual heart (round dot, WT with DMSO injection group; frame dot, Tg(∆113p53:cmyc) with DMSO injection group; triangle dot, Tg(∆113p53:cmyc) with WZB117 injection group). n: 6–7 hearts/sample. The experiment was repeated two times. (D) Statistical analyses of scar areas in (B). Average injury area without MF20 signals on heart sections was presented as the percentage of the total ventricular area. Each dot represents an individual heart (round dot, WT with DMSO injection group; frame dot, Tg(∆113p53:cmyc) with DMSO injection group; triangle dot, Tg(∆113p53:cmyc) with WZB117 injection group). n: 6–7 hearts/sample. The experiment was repeated two times. Statistical analysis was performed by Student’s two-tailed unpaired t test in GraphPad Prism 8. The p values were represented by n.s. and asterisks. *, p < 0.05; **, p < 0.01.
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