Jag2b regulates efna1b expression in the somites. (A) Expression of efna1b in wild-type and jag2bsgRNA embryos. Black arrowheads indicate efna1b expression in the somites. Graph shows the distribution of embryos exhibiting ‘high’, ‘middle’ or ‘low’ runx1 or wnt16 expression phenotypes. (B) Expression of efnb2a in the somites of wild-type or jag2bsgRNA embryos. (C-G) Time course of efna1b expression in wild-type embryos. (H-J) Expression of efna1b, Tp1:GFP or both in the somites at 14 hpf. White arrowheads indicate cells expressing efna1b, Tp1:GFP or both. A high-magnification view of the boxed region in J is shown to the right. (K) Expression of wnt16 (green) and efna1b (red) in the somites at 15 hpf. Dorsal views of the embryo are shown. White arrowheads indicate instances of an efna1b-expressing cell adjacent to a wnt16-expressing cell. (L,M) Expression of runx1 in the DA and wnt16 in the somite of wild-type and efna1bsgRNA embryos. Black arrowheads indicate runx1 expression in the DA (L) and wnt16 expression in the somites (M). Graphs show the distribution of embryos exhibiting ‘high’, ‘middle’ or ‘low’ runx1 or wnt16 expression phenotypes. *P<0.05; ***P<0.001. Scale bars: 200 μm (A-G,M); 10 μm (H-J); 20 μm (K); 100 μm (L).
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